Part:BBa_K1727007

T5+Lac Operator+RBS+signal peptide+Epinecidin-1+Terminater

Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies. In order to have more efficient to get Epinecidin-1, we treated signal peptide from S.lividans on the upstream of N-terminal of antimicrobial peptides to facilitate the peptide production. This part is built by part:BBa_K1727003.

We used T5 promoter, Lac operator, RBS, terminator on pQE 60 vector. So we can use IPTG induction to get our product.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 79
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 79
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 79
  Illegal BglII site found at 415
  Illegal BamHI site found at 112
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 79
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 79
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 358